Nifurpirinol: A potent inducer of FSGS-like glomerular injury in transgenic zebrafish
Klawitter, M.; Siegerist, F.; Daniel, S.; Schindler, M.; Endlich, N.
Show abstract
Identifying effective drugs for focal segmental glomerulosclerosis (FSGS) treatment holds significant importance. Our high-content drug screening on zebrafish larvae relies on nitroreductase/metronidazole (NTR/MTZ)-induced podocyte ablation to generate FSGS-like injury. A crucial factor for successful drug screenings is minimizing variability in injury induction. For this, we introduce Nifurpirinol (NFP) as a more reliable prodrug for targeted podocyte depletion. NFP showed a 2.3-fold increase in efficiency at concentrations 1600-fold lower compared to MTZ-mediated injury induction. Integration into the screening workflow validated its suitability for the high-content drug screening. The presence of crucial FSGS hallmarks, including podocyte foot process effacement, proteinuria, and activation of parietal epithelial cells, was observed. In this study, we demonstrate that NFP serves as remarkably effective prodrug for inducing an FSGS-like phenotype in zebrafish larvae and proves to be well-suited for a high-content drug screening, aiding in the identification of potential candidates for FSGS treatment.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Correlation of calcium multiphoton with ultrastructural STED imaging of the slit diaphragm in the same glomerulus. 94%
- Creation of X-linked Alport Syndrome Rat Model with Col4a5 Deficiency 94%
- Piezo activity levels need to be tightly regulated to maintain normal morphology and function in pericardial nephrocytes 94%
Similar papers in this journal
- An amino-terminal fragment of apolipoprotein E4 leads to behavioral deficits, increased PHF-1 immunoreactivity, and mortality in zebrafish 94%
- Genetic Deletion of Calcium-independent Phospholipase A2γ Protects Mice from Diabetic Nephropathy 94%
- Recombinant human soluble thrombomodulin is associated with attenuation of sepsis-induced renal impairment by inhibition of extracellular histone release 93%
Similar papers in this journal
- Combining supervised and unsupervised analyses to quantify behavioral phenotypes and validate therapeutic efficacy in a triple transgenic mouse model of Alzheimer's disease 91%
- JQ-1 ameliorates schistosomiasis liver fibrosis by suppressing JAK2 and STAT3 activation 91%
- siRNA-based Therapeutic Candidate Targeting PRDM2 for Inhibition of Lung Cancer Progression 91%
Similar papers in this journal
- Derivation of the immortalized cell line-UM51-PrePodo-hTERT and its responsiveness to Angiotensin II and activation of RAAS 93%
- ELOVL2 is required for robust visual function in zebrafish 92%
- Human iPSC-derived renal cells change their immunogenic properties during maturation: Implications for regenerative therapies 92%
Similar papers in this journal
- A zebrafish model for studying mechanisms of newborn hyperbilirubinemia and bilirubin induced neurological damage 93%
- Artesunate Alleviate Kidney Fibrosis by Restoring Klotho Protein and Suppressing Wnt/β-Catenin Signalling Pathway 92%
- Coagulation Factor IIIa (f3a) Knockdown in Zebrafish Leads to Defective Angiogenesis and Mild Bleeding Phenotype 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.