An amplicon-based Illumina and nanopore sequencing workflow for Chikungunya virus West Africa genotype.
Dieng, I.; Ndiaye, M.; Kane, M.; Balde, D.; Mbanne, M.; Diop, S. M. B. S.; Sankhe, S.; Dia, M.; Dieng, M.; Doukanda, S. F. M.; Faye, O.; Sall, A. A.; Dia, N.; Fall, G.; Faye, O.; Diagne, M. M.
Show abstract
The Chikungunya virus, a global arbovirus, is currently causing a major outbreak in the Western African region, with the highest cases reported in Senegal and Burkina Faso. Recent molecular evolution analyses reveal that the strain responsible for the epidemic belongs to the West African genotype, with new mutations potentially impacting viral replication, antigenicity, and host adaptation. Real-time genomic monitoring is needed to track the viruss spread in new regions. A scalable West African genotype amplicon-based Whole Genome Sequencing for multiple Next Generation Sequencing platforms has been developed to support genomic investigations and identify epidemiological links during the viruss ongoing spread. This technology will help identify potential threats and support real-time genomic investigations in the ongoing spread of the virus.
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