Inhibition of miR-199b-5p reduces pathological alterations in Osteoarthritis by potentially targeting Fzd6 and Gcnt2.
Feng, T.; Zhang, Q.; Li, S.-H.; ping, y.-l.; tian, M.-q.; Zhou, S.-h.; Wang, X.; Wang, J.-M.; Liang, F.; Yu, S.-G.; Wu, Q.
Show abstract
Osteoarthritis (OA) is a degenerative disease with a high prevalence in the elderly population, but our understanding of its mechanisms remains incomplete. Analysis of serum exosomal small RNA sequencing data from clinical patients and gene expression data from OA patient serum and cartilage obtained from the GEO database revealed a common dysregulated miRNA, miRNA-199b-5p. In vitro cell experiments demonstrated that miRNA-199b-5p inhibits chondrocyte vitality and promotes extracellular matrix degradation. Conversely, inhibition of miRNA-199b-5p under inflammatory conditions exhibited protective effects against damage. Local viral injection of miRNA-199b-5p into mice induced a decrease in pain threshold and OA-like changes. In an OA model, inhibition of miRNA-199b-5p alleviated the pathological progression of OA. Furthermore, bioinformatics analysis and experimental validation identified Gcnt2 and Fzd6 as potential target genes of miRNA-199b-5p. Thus, these results indicated that miRNA-199b-5p/Gcnt2 and Fzd6 axis might be a novel therapeutic target for the treatment of OA.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Aberrant methylation and expression of TNXB promote chondrocyte apoptosis and extracullar matrix degradation in hemophilic arthropathy via AKT signaling 98%
- GLI1 facilitates rheumatoid arthritis by collaborative regulation of DNA methyltransferases 96%
- Down-regulated GAS6 impairs synovial macrophage efferocytosis and promotes obesity-associated osteoarthritis 96%
Similar papers in this journal
- MicroRNA-378 suppressed osteogenesis of mesenchymal stem cells and impaired bone formation via inactivating Wnt/β-catenin signaling 95%
- MiR-125b differentially impacts mineralization in dexamethasone and calcium-treated human mesenchymal stem cells 92%
- Exploring Adenosine Analogues for Chondrosarcoma Therapy: In Vitro and In Vivo Insights 91%
Similar papers in this journal
- Aging-associated Increase of GATA4 levels in Articular Cartilage is Linked to Impaired Regenerative Capacity of Chondrocytes and Osteoarthritis 95%
- Combining segmental bulk- and single-cell RNA-sequencing to define the chondrocyte gene expression signature in the murine knee joint 95%
- BMP signaling: A significant player and therapeutic target for osteoarthritis 94%
Similar papers in this journal
- Autologous iPSC- and MSC-derived Chondrocyte Implants for Cartilage Repair in a Miniature Pig Model 93%
- Emodin-Enhanced hUC-MSC Extracellular Vesicles Alleviate Acute Pancreatitis by Targeting Inflammation and Pyroptosis 92%
- Interactions among Merlin, Arkadia, and SKOR2 mediate NF2-associated Schwann cell proliferation in human. 92%
Similar papers in this journal
- Rcn3 is Involved in Postnatal Tendon Development by Regulating Collagen Modification and Fibrillogenesis 93%
- Multi-omics profiling of collagen-induced arthritis mouse model reveals early metabolic dysregulation via SIRT1 axis 93%
- Identification of Sensory Fiber Types in Mouse Temporomandibular Joint Tissues 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.