Promoting intestinal healing by exclusive enteral nutrition with TGF-beta in a mouse model of colitis.
Boumessid, K.; Lacroix, V.; Ovtchinnikova, E.; Quaranta-Nicaise, M.; Roy, M.; Dumay, A.; Thenet, S.; Carriere, M.; Mas, E.; Barreau, F.
Show abstract
Background and aimsExclusive Enteral Nutrition (EEN) is the first line of treatment for pediatric Crohns disease (CD), but its mechanisms of action remain poorly understood. We studied EEN nutritional composition and TGF-{beta} effect in a mouse model of colitis, as well as the role of intestinal microbiota. MethodsMice were treated with Dextran Sulfate Sodium (DSS) during 5 days to induce colitis, until the inflammatory peak (day 7) or gut restitution (day 14). After DSS treatment, some of them received EEN formula such as Modulen IBD(R) (DM mice) or Infatrini Peptisorb(R) (DINF mice), with TGF-{beta} supplementation or neutralization, and clinical inflammation was evaluated. After sacrifice, macroscopic and microscopic inflammation were analyzed, as well as intestinal permeability (IP). The composition of mucosal colonic microbiota was analyzed and fecal microbiota transplantation was performed to evaluate its capacity to mediate anti-inflammatory and pro-regenerative effect. Colonic crypts from DSS and EEN mice were cultured as 3D organoids and cellular properties were analyzed. ResultsDSS mice developed colitis, as evidenced by the weight loss and clinical inflammation. It was accompanied by macroscopic inflammation such as colon thickness and edemas, and an elevated IP. In contrast, EEN mice with TGF-{beta} formula present faster weight recovery and decreased inflammatory parameters, with a normalized IP, suggesting gut restitution and functionality. These functional improvements were not obtained for EEN mice without TGF-{beta} formula. Moreover, EEN with Modulen IBD(R) (DM mice) modified the microbiota in comparison to DSS condition and attenuated inflammation. In addition, the organoids from DM mice colonic crypts treated had an enhanced survival, and re-epithelialization capacity. ConclusionsBoth EEN formula have anti-inflammatory properties, certainly by the nutritional composition. However, TGF-{beta} plays a significant role in intestinal restitution and restoring barrier function. These beneficial effects are partly mediated by the microbiota to maintain gut homeostasis.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Loss of intestinal endosome associated protein sorting nexin 27 disrupts epithelial barrier and promotes inflammation 97%
- The appendix orchestrates T-cell mediated immunosurveillance in colitis-associated cancer 96%
- Metagenomic alterations in gut microbiota precede and predict onset of colitis in the IL10 gene-deficient murine model 95%
Similar papers in this journal
- Enhancing Recovery from Gut Microbiome Dysbiosis and Alleviating DSS-Induced Colitis in Mice with a Consortium of Rare Short-Chain Fatty Acid-Producing Bacteria 96%
- An IBD-associated pathobiont synergises with NSAID to promote colitis which is blocked by NLRP3 inflammasome and Caspase-8 inhibitors 96%
- Deprivation of dietary fiber in specific-pathogen-free mice promotes susceptibility to the intestinal mucosal pathogen Citrobacter rodentium 94%
Similar papers in this journal
- Intestinal barrier function in the naked mole-rat: an emergent model for gastrointestinal insights 95%
- A Leaky Human Colon Model Reveals Uncoupled Apical/Basal Cytotoxicity in Early Clostridioides difficile Toxin Exposure 94%
- Fecal microbiota transplantation mitigates cardiac remodeling and functional impairment in mice with chronic colitis 94%
Similar papers in this journal
Similar papers in this journal
- Intestinal Bacteria Maintain Adult Enteric Nervous System and Nitrinergic Neurons via Toll-like Receptor 2-induced Neurogenesis in Mice 94%
- Reduced expression of COVID-19 host receptor, ACE2 is associated with small bowel inflammation, more severe disease, and response to anti-TNF therapy in Crohn's disease 94%
- Early-life microbial intervention reduces colitis risk promoted by antibiotic-induced gut dysbiosis 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.