Achatina fulica Haemocyanin-Derived Peptides as Novel Antimicrobial Agents
Pereira Patino, A. E.; Suarez Largo, L. A.; Roman, T.; Guzman Quimbayo, F.; Rincon-Orozco, B.; Sierra Guevara, L. T.; Hidalgo Bucheli, W. F.
Show abstract
Anteriorly we found Haemocyanin-derived peptides in semi-purified fractions of mucus secretion of Achatina fulica that showed an inhibitory effect on S. aureus strains. Here, we applied in silico rational design strategy to obtain new potential Antimicrobial Peptides (AMPs) from A. fulica haemocyanin-derived peptides (AfH). Designed peptides were chemically synthetized using the Fmoc strategy and antimicrobial activity against E. coli and S. aureus strains was investigated using the broth microdilution method. Cytotoxic activity on Vero, HaCat, and human erythrocytes cells was also evaluated. The results showed that 15-residue synthetic peptides, alpha-helical and cationic, were those with the highest biological activity against Gram positive strains, with Minimum Inhibitory Concentrations (MIC) in the range of 20 to 30 {micro}M. The positive selectivity index suggests a higher selectivity mainly on the microorganisms evaluated and not on eukaryotic cells. In this study, A. fulica hemocyanin turned out to be an appropriate protein model for the rational design of AMPs against bacteria of public health importance. Additional studies are required to evaluate the activity of the peptides on Gram negative bacteria.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Mode of action of the antimicrobial peptide Mel4 is independent of Staphylococcus aureus cell membrane permeability 96%
- The inhibitory effects of toothpaste and mouthwash ingredients on the interaction between the SARS-CoV-2 spike protein and ACE2, and the protease activity of TMPRSS2, in vitro 95%
- Comprehensive Genome Based Analysis of Vibrio parahaemolyticus for Identifying Novel Drug and Vaccine Molecules: Subtractive Proteomics and Vaccinomics Approach 95%
Similar papers in this journal
- HPMCP-coated microcapsules containing the Ctx(Ile21)-Ha antimicrobial peptide reduces the mortality rate caused by resistant Salmonella Enteritidis in poultry 96%
- AntiBP3: A hybrid method for predicting antibacterial peptides against gram-positive/negative/variable bacteria 94%
- Capsaicin potently blocks Salmonella typhimurium invasion of Vero cells 94%
Similar papers in this journal
Similar papers in this journal
- Neutralizing effects of small molecule inhibitors and metal chelators on coagulopathic Viperinae snake venom toxins 93%
- Bioengineered ciprofloxacin loaded chitosan nanoparticles for the treatment of Bovine Mastitis 91%
- Rapid and accurate discrimination of Mycobacterium abscessus subspecies based on matrix-assisted laser desorption ionization-time of flight spectrum and machine learning 90%
Similar papers in this journal
- Macrolactin A Is an Inhibitor of Protein Biosynthesis in Bacteria 95%
- Structural modeling and thermostability of a serine protease inhibitor belonging to the Kunitz family from the tick Rhipicephalus microplus 94%
- The β-lactam Ticarcillin is a Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase binder. 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.