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Systematic Characterization of LSD metabolites in C. elegans by ultra-high performance liquid chromatography coupled with high-resolution tandem mass spectrometry

Silveira, C. M. d. V.; dos Santos, V. F.; Ornelas, I. M.; Carrilho, B. d. S.; Ventura, M. A. V. d. C.; Pereira, H. M. G.; Rehen, S. K.; Junqueira, M.

2023-06-21 pharmacology and toxicology
10.1101/2023.06.19.545563 bioRxiv
Show abstract

Psychedelic compounds have gained renewed interest for their potential therapeutic applications, but their metabolism and effects on complex biological systems remain poorly understood. Here, we present a systematic characterization of LSD metabolites in the model organism Caenorhabditis elegans using state-of-the-art analytical techniques. By employing ultra-high performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), we identified and quantified a range of LSD metabolites, shedding light on their metabolic pathways and offering insights into their pharmacokinetics. Our study demonstrates the suitability of C. elegans as a valuable model system for investigating the metabolism of psychedelic compounds and provides a foundation for further research on the therapeutic potential of LSD. These findings contribute to the growing body of knowledge in the field and highlight the importance of advanced analytical methodologies in elucidating the effects of psychedelic substances on biological systems.

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