Expression and molecular regulation of key genes during adipogenesis of neural crest stem cells
Dong, B.; Guan, W.-c.; Zhang, K.; Zhang, Y.; Yang, Y.; Zhao, Y.-p.; Bai, R.; Zheng, M.-x.; Cui, X.
Show abstract
Neural crest stem cells (NCSCs) are pluripotent stem cells derived from the "fourth germ layer". Similar to mesenchymal stem cells (MSCs), NCSCs can differentiate into a variety of cell types, such as adipocytes. However, the mechanism of NCSCs adipogenesis remains unclear. Previously, we have revealed that primary cells have neural crest and stem cell properties and can differentiate into adipocytes. Therefore, in this study, the differentially expressed genes (DEGs) of NCSCs at specific time points of adipogenesis were predicted by mRNA sequencing, the key genes of adipogenesis were predicted by principal component analysis, heat map, GO and KEGG enrichment analysis, and the expression of DEGs was verified by RT-qPCR. RNA interference (RNAi) technology was used to inhibit the expression of DEGs, and RT-qPCR and Western blot were used to explore the regulatory mechanism between DEGs. Sequencing results indicated a possible regulatory relationship between C/EBP, C/EBP{beta}, C/EBP{delta} and PPAR{gamma}. The results of RT-qPCR were consistent with those of mRNA sequencing. Combined with RT-qPCR and western blot results, we found that C/EBP{beta} and PPAR{gamma} regulated the transcription of C/EBP during NCSCs adipogenesis, and C/EBP{beta} and PPAR{gamma} formed positive feedback loop.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The up-regulation of TGF-beta1 by miRNA-132-3p/WT1 is involved in inducing leukemia cells to differentiate into macrophages 97%
- Decorin inhibits glucose-induced lens epithelial cell apoptosis via suppressing p22phox-p38 MAPK signaling pathway 97%
- DHA but not EPA induces the trans-differentiation of C2C12 cells into white-like adipocytes phenotype 96%
Similar papers in this journal
- Ginsenoside Rg1 defenses PC-12 cells against hydrogen peroxide-caused damage via up-regulation of miR-216a-5p 96%
- Metformin prevented high glucose-induced endothelial reactive oxygen species via OGG1 in an AMPKα-Lin-28 dependent pathway 95%
- TRPV4 modulation affects mitochondrial parameters in adipocytes and its inhibition upregulates lipid accumulation 94%
Similar papers in this journal
- The protective roles of Eugenol on type 1 diabetes mellitus through NRF2 mediated oxidative stress pathway 95%
- Interleukin-33 stimulates stress in the endoplasmic reticulum of the human myometrium via an influx of calcium during initiation of labor 95%
- Fibroblast activation during decidualization: Embryo-derived TNFα induction of PGI2-PPARδ-ACTIVIN A pathway through luminal epithelium 94%
Similar papers in this journal
- Effects of different environmental intervention durations on the intestinal mucosal barrier and the brain-gut axis in rats with colorectal cancer 94%
- RT-qPCR analyses on the osteogenic differentiation from human iPS cells: An investigation of reference genes 94%
- Effect of mechanical stretching stimulation on maturation of human iPS cell-derived cardiomyocytes co-cultured with human gingival fibroblasts 94%
Similar papers in this journal
- Anti-nucleolin aptamer, iSN04, inhibits the inflammatory responses in myoblasts by modulating the β-catenin/NF-κB signaling pathway 94%
- Angiotensin II induces apoptosis in human induced pluripotent stem cell-derived cardiomyocytes 94%
- Development of a Novel Japanese Eel Myoblast Cell Line for Application in Cultured Meat Production 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.