Thioflavin T In-gel Stain to Study Protein Misfolding in Frozen Tissue Specimens
Oldam, J.; Tchernyshyov, I.; Van Eyk, J.; Troncoso, J.; Glabe, C. G.; Agnetti, G.
Show abstract
There are limited options to quantify and characterize amyloid species from biological samples in a simple fashion. Thioflavin T (ThT) has now been used for decades to stain amyloid fibrils but to our knowledge we were the first to use it in-gel. Thioflavin T in-gel stain is convenient as it is fast, inexpensive, available to most labs, compatible with other fluorescent stains and downstream analyses such as mass spectrometry (MS).
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