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Serum from pregnant donors induces human beta cell proliferation and insulin secretion

Sylvester-Armstrong, K. R.; Reeder, C. F.; Powell, A.; Becker, M. W.; Hagan, D. W.; Chen, J.; Mathews, C. E.; Wasserfall, C. H.; Atkinson, M. A.; Egerman, R.; Phelps, E. A.

2023-04-17 cell biology
10.1101/2023.04.17.537214 bioRxiv
Show abstract

Pancreatic beta cells are among the slowest replicating cells in the human body. Human beta cells usually do not increase in number with exceptions being during the neonatal period, in cases of obesity, and during pregnancy. This project explored maternal serum for stimulatory potential on human beta cell proliferation and insulin output. Gravid, full-term women who were scheduled to undergo cesarean delivery were recruited for this study. A human beta cell line was cultured in media supplemented with serum from pregnant and non-pregnant donors and assessed for differences in proliferation and insulin secretion. A subset of pregnant donor sera induced significant increases in beta cell proliferation and insulin secretion. Pooled serum from pregnant donors also increased proliferation in primary human beta cells but not primary human hepatocytes indicating a cell-type specific effect. This study suggests stimulatory factors in human serum during pregnancy could provide a novel approach for human beta cell expansion.

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