Cytotrophoblast cells are selectively permissive and favor Zika virus, but no other related flavivirus, invasion to the placental stroma
Viettri, M. I.; Caraballo, G. I.; Sanchez, M. E.; Espejel-Nunez, A.; Betanzos, A.; Ortiz-Navarrete, V.; Estrada, G.; Nava, P.; Ludert, J. E.
Show abstract
BACKGROUNDZika virus (ZIKV) is highly teratogenic, in contrast with dengue virus (DENV) or the yellow fever virus vaccine (YFV-17D). The mechanisms employed by ZIKV to cross the placenta need to be elucidated. METHODSParallel infections with ZIKV, DENV and YFV-17D were compared in terms of efficiency, activation of mTOR pathways and cytokine secretion profile in human cytotrophoblastic HTR8 cells (CTB), and monocytic U937 cells, differentiated to M2 macrophages (M2-MO). RESULTSIn CTB, ZIKV replication was significantly more efficient than DENV or YFV-17D. In M2-MO, ZIKV replication continued to be more efficient, although differences between strains were reduced. Significantly greater activation of Phospho-S6r and Phospho-AKT/Ser473 fractions in CTB infected with ZIKV than with DENV or YFV-17D, was observed. CTB treated with the mTOR inhibitors rapamycin or AZD8055, showed a 20-fold-reduction in ZIKV yield, versus 5 and 3.5-fold for DENV and YFV-17D, respectively. Finally, we detected that ZIKV infection, but not DENV or YFV-17D, efficiently inhibited the interferon response of CTB cells. CONCLUSIONSThese results suggest that CTB cells are permissive and act favoring ZIKV entry into the placental stroma, over DENV and YFV-17D and that the mTOR complex is a switch that enhances the replication of ZIKV in CTB cells.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Mutation nsp6 L232F associated with MERS-CoV zoonotic transmission confers higher viral replication in human respiratory tract cultures ex-vivo 95%
- Type I Interferon acts as a major barrier to the establishment of infectious bursal disease virus (IBDV) persistent infections 95%
- Species-specific molecular barriers to SARS-CoV-2 replication in bat cells 95%
Similar papers in this journal
- Assessing Human Liver Spheroids as a Model for Antiviral Drug Evaluation Against BSL-3 Haemorrhagic Fever viruses 96%
- Development and optimization of biologically contained Marburg virus for high-throughput antiviral screening 95%
- Defining functional neuraminidase inhibitor drug resistance motifs in avian influenza viruses and the consequential impact on virus fitness in chicken cells. 94%
Similar papers in this journal
- TREM-1 activation is a key regulator in driving severe pathogenesis of enterovirus 71 infection 95%
- Generation of OP7 chimera defective interfering particle preparations free of infectious influenza A virus that shows antiviral efficacy in mice 95%
- Replication of Equine arteritis virus is efficiently suppressed by purine and pyrimidine biosynthesis inhibitors 95%
Similar papers in this journal
- The astrovirus N-terminal nonstructural protein anchors replication complexes to the perinuclear ER membranes 95%
- Repression of varicella zoster virus gene expression during quiescent infection in the absence of detectable histone deposition 95%
- A role of hypoxia inducible factor 1 alpha in Mouse Gammaherpesvirus 68 (MHV68) lytic replication and reactivation from latency 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.