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In-vitro Analysis of Hemostatic Cotton Khadi Dressing with Kaolin/Chitosan-based Composition and Its Application as Prospective Wound Dressing

Raval, A. A.; Patel, V. N.; Pancholi, U. V.

2022-08-15 bioengineering
10.1101/2022.08.12.503747 bioRxiv
Show abstract

The current investigation illustrates the formulation of Kaolin/Chitosan-based composition by using a simple mechanochemical method that can produce a stabilized hemostatic composition. Chitosan is stabilized in sodium hydroxide solution with which kaolin solution is mixed under controlled conditions. When the formulated hemostatic composition was impregnated on cotton khadi dressing it showed drastically improved hemostatic efficiency as compared to standard cotton gauze. The tightly woven cotton khadi fabric has a larger surface area as compared to standard cotton gauze and so it retains more hemostatic composition. The hemostatic properties of the composition are characterized using various in-vitro testing techniques like bleeding time analysis, clotting time analysis, absorption strength study, and several other parameters. Investigation of the freshly drawn blood samples from humans was used for experimenting in order to obtain highly accurate results. The results demonstrate that Kaolin/Chitosan composite impregnated cotton khadi dressing can noticeably enhance the hemostasis other than being biocompatible as demonstrated through an animal model and fresh blood study.

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