SIC50: Determination of IC50 by an optimized Sobel operator and a vision transformer
Wang, Y.; Zhang, W.; Yip, H.; Qu, C.; Hu, H.; Chen, X.; Lee, T.; Yang, X.; Yang, B.; Kumar, P.; Lee, S. Y.; Casimiro, J.; Zhang, J.; Lam, K. S.; Wang, A.
Show abstract
As a measure of cytotoxic potency, half-maximal inhibitory concentration (IC50) is the concentration at which a drug exerts half of its maximal inhibitory effect against target cells. It can be determined by various methods that require applying additional reagents or lysing the cells. Here, we describe a label-free Sobel-edge-based method, which we name SIC50, for the evaluation of IC50. SIC50 classifies pre-processed phase-contrast images with a state-of-art vision transformer and allows for the continuous assessment of IC50 in a faster and more cost-efficient manner. We have validated this method using four drugs and 1536-well plates, as well as built a first-of-its-kind web application. We anticipate this method will assist in the high-throughput screening of chemical libraries (e.g., small molecule drugs, siRNA, and microRNA and drug discovery.
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