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The AppNL-G-F mouse model of Alzheimer's disease is refractory to regulatory T cell treatment

Yshii, L.; Mascali, L.; Kouser, L.; Lemaitre, P.; Marino, M.; Dooley, J.; Burton, O.; Haughton, J.; Callaerts-Vegh, Z.; de Strooper, B.; Holt, M.; Pasciuto, E.; Liston, A.

2022-03-14 immunology
10.1101/2022.03.11.483903 bioRxiv
Show abstract

BackgroundAlzheimers Disease is a neurodegenerative disease with a neuroinflammatory component. Due to the multifunctional capacity of regulatory T cells to prevent and reverse inflammation, regulatory T cells have been proposed as a potential therapeutic in Alzheimers Disease, either as a direct cell therapy or through the use of IL2 as a biologic to expand the endogenous population. MethodsHere we characterize the longitudinal immunological changes occurring in T cells in the AppNL-G-F mouse model of Alzheimers disease. ResultsAge-dependent immunological changes, in both the brain and periphery, occurred in parallel in both AppNL-G-F mice and control AppNL mice. As the endogenous IL2 axis was disturbed with age, we sought to determine the effect of IL2 supplementation on disease progression. Using a genetic model of IL2 provision in the periphery or in the brain, we found that expanding regulatory T cells in either location was unable to alter the progression of key pathological events or behavioral changes. ConclusionThese results suggest that either the AppNL-G-F mouse model does not recapitulate key regulatory T cell-dependent process of Alzheimers disease, or that regulatory T cell therapy is not a promising candidate for APP-mutation-driven Alzheimers disease.

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