Amplicon-based nanopore minion sequencing of patients with COVID-19 omicron variant from India
Kumar, S.; Lomash, A.; Faruq, M.; Siddiqui, O.; Kumar, S.; v, S.; Suravajhala, P.; Polipalli, S. K.; SCOG_MAMC_ LNH,
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SARS-CoV-2 infection has been playing havoc with emerging omicron variants of concern (VoC). Here, we report sequencing of the omicron variant in 13 patients from India using Oxford Nanopore Technology (ONT) Minion, wherein a rapid amplicon based sequence analysis was performed to assess and compare with existing 34 mutations in spike glycoprotein. We highlight and discuss the nature of these mutations that are unique and common to other populations. This is perhaps the first report on omicron variants from India using a long read sequencing chemistry.
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