S Gene Target Failure (SGTF) in Commercial Multiplex RT-PCR assay as indicator to detect SARS-CoV-2 VOC B.1.1.7 lineage in Tamil Nadu, India
N M, V.; A, K.; V, K.; A, R. K.; Subramanian, G.; Rathinasamy, A. S.; Ramakrishnan, U.; Potdar, V. A.; Sivadoss, R.; P, S.; T S, S.
Show abstract
Emergence of Severe Acute Respiratory Syndrome Corona Virus-2 (SARS-CoV-2) Variants of Concern (VOC) possessing improved virulence, transmissibility and/or immune-escape capabilities has raised significant public health concerns. In order to identify VOCs, WHO recommends Whole-Genome Sequencing approach, which is costly and involves longer completion time. Hence, potential role of commercial multiplex RT-PCR kit to screen variants rapidly is being attempted in this study. A total of 1200 suspected COVID samples from different districts of Tamil Nadu State (India) were screened with Thermo TaqPath RT-PCR kit and Altonas Realstar RT-PCR Assay kit. Among 1200 screened, S-gene target failure (SGTF) phenomenon were identified in 112 samples while testing with TaqPath RT-PCR Kit. 100% concordant results were observed between SGTF phenomenon and whole-genome sequencing (WGS) results in detecting SARS-CoV-2 VOC B.1.1.7. TaqPath RT-PCR assay testing can be utilized by laboratories to screen rapidly the VOC B.1.1.7 variants, thus enabling early detection of B.1.1.7 variant infection and transmission in population. This in turn will pave way to implement suitable preventive measures by appropriate authorities to control the transmission of the viral variant.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Evaluating diagnostic accuracies of Panbio ™ COVID-19 rapid antigen test and RT-PCR for the detection of SARS-CoV-2 in Addis Ababa, Ethiopia using Bayesian Latent-Class Models (BLCM) 96%
- SARS-CoV-2 detection in multi-sample pools in a real pandemic scenario: a screening strategy of choice for active surveillance 96%
- Development and Evaluation of Two Rapid Indigenous IgG-ELISA immobilized with ACE-2 Binding Peptides for Detection Neutralizing Antibodies Against SARS-CoV-2 95%
Similar papers in this journal
- An Upsurge of SARS CoV-2 B.1.1.7 Variant in Pakistan 97%
- Seroprevalence of SARS-CoV-2 infection in the Colombo Municipality region, Sri Lanka 95%
- Profiles of bacteria isolates and their antimicrobial resistance pattern among housemaids working in communal living residences in Jimma City, Ethiopia 94%
Similar papers in this journal
- Performance of SARS-CoV-2 rapid antigen test compared with real-time RT-PCR in asymptomatic individuals 95%
- Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction 94%
- Self-collected oral, nasal and saliva samples yield sensitivity comparable to professional-collected oro-nasopharyngeal swabs in SARS-CoV-2 diagnosis 94%
Similar papers in this journal
Similar papers in this journal
- Genomic diversity of SARS-CoV-2 in Pakistan during fourth wave of pandemic 95%
- A rapid and cost-effective multiplex ARMS-PCR method for the simultaneous genotyping of the circulating SARS-CoV-2 phylogenetic clades 95%
- Upsurge in Hospitalization of Pediatric Patients with Severe Acute Respiratory Infections in Kolkata and Surrounding Districts Caused by Recombinant Human Respiratory Adenovirus Type B 7/3 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.