Vectofusin-1 based T-cell transduction approach for developing murine CAR-T cells for cancer.
Patel, R.; Devashish, K.; Singh, S.; Nath, P. R.; Gohel, D. M.; Prasad, R.; Singh, M.; Saini, N. Y.
Show abstract
Gene transfer into human and murine T-cells using viral-based approaches has several promising therapeutic applications including the production of chimeric antigen receptor T-cell (CAR-T) therapy. The generation of murine CAR-T is paramount to test and validate immunocompetent mouse models for CAR-T therapy. Several viral transduction enhancers already exist for gene therapy with few limitations. In this study, we tested vectofusin-1, a short cationic peptide, as a soluble transduction enhancer for gammaretroviral transduction for the generation of anti-CD19 murine CAR-T. We found that in comparison to Retronectin, Vectofusin-1 is an equally optimal transduction enhancer for the generation of murine CAR-T cells.
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