Analysis, quantification, and visualization of RT-LAMP technique for detection of COVID-19
Ghazimoradi, M. H.; Daryani, M.; Gharshasbi, M.; Zolghadr, E.; Khalafizadeh, A.; Babashah, S.
Show abstract
BackgroundSARS-Cov-2 is a new virus that caused an epidemic disease, COVID-19. According to the world health organization, detecting the patients/carriers is by the far the most important action to prevent the pandemic. Recently, the loop-mediated isothermal amplification (LAMP) technique has become more popular due to the easy handling of a one-step kit used for the detection of many diseases than RT-PCR-based techniques. methodsHerein, we used the RT-LAMP technique so as to detect COVID-19. To this end, 40 paired-samples of patients and healthy people had been collected and tested by RT-PCR for N and E genes of SARS-CoV-2. The RT-LAMP test has been performed on samples for the RdRp gene. The sensitivity and specificity of tests have been determined. ResultsThe testing results are consistent with the conventional RT-qPCR. Additionally, we also showed that a one-step process without RNA extraction is feasible to achieve RNA amplification directly from a sample. ConclusionWe confirmed that RT-LAMP is a rapid, simple, and sensitive method that can be used as a large-screening method, particularly in regional hospitals with limited access to high-technologies.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Detection of SARS-CoV-2 in Different Human Biofluids Using the Loop-Mediated Isothermal Amplification Assay: A Prospective Diagnostic Study in Fortaleza, Brazil 97%
- Evaluation of seven different rapid methods for nucleic acid detection of SARS-COV-2 virus 96%
- A rapid and cost-effective multiplex ARMS-PCR method for the simultaneous genotyping of the circulating SARS-CoV-2 phylogenetic clades 96%
Similar papers in this journal
- A bioinformatics approach to systematically analyze the molecular patterns of monkeypox virus-host cell interactions 94%
- Retrospective and prospective studies evaluating the performance of the SARS-Cov-2 “AQ+ COVID-19 Ag Rapid Test” from InTec on symptomatic and non-symptomatic patients 93%
- A small H2O-soluble ingredient of royal jelly lower cholesterol levels in liver cells by suppressing squalene epoxidase 93%
Similar papers in this journal
- Validation of a Novel Molecular Assay to the Diagnostic of COVID-19 Based on Real Time PCR with High Resolution Melting 97%
- SARS-CoV-2 detection in multi-sample pools in a real pandemic scenario: a screening strategy of choice for active surveillance 96%
- High throughput SARS-CoV-2 variant analysis using molecular barcodes coupled with Next Generation Sequencing 95%
Similar papers in this journal
- Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis 98%
- A Paper-based Loop-Mediated Isothermal Amplification (LAMP) Assay for Highly Pathogenic Avian Influenza 96%
- Design of Specific Primer Set for Detection of B.1.1.7 SARS-CoV-2 Variant using Deep Learning 96%
Similar papers in this journal
- Rapid and simultaneous identification of three mutations by the Novaplex™ SARS-CoV-2 Variants I Assay kit 96%
- Evaluation of three rapid lateral flow antigen detection tests for the diagnosis of SARS-CoV-2 infection 95%
- Is the glass half full? Extraction-free RT-LAMP to detect SARS-CoV-2 is less sensitive but highly specific compared to standard RT-PCR in 101 samples 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.