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Lung Epithelial Cells Can Produce Antibodies Participating In Adaptive Humoral Immune Responses

Gao, E.; Shao, W.; Zhang, C.; Yu, M.; Dai, H.; Fan, T.; Zhu, Z.; Xu, W.; Huang, J.; Zhang, Y.; Qin, Z.; Qiu, X.

2021-05-15 immunology
10.1101/2021.05.13.443498 bioRxiv
Show abstract

It is generally believed that the main source of antibodies is B lymphocytes. In this study, our results first revealed that B cell-deficient mice could not produce antibodies specific for TI-Ags; however, mice with B cell deficiency could produce TD-Ag-specific antibodies, although antibody production was delayed compared with that in BALB/c mice after primary TD-Ag challenge. Subsequently, we identified that mouse lung epithelial cells could produce and secrete Ig, including IgM, IgA or IgG, which could display TD-Ag-specific antibody activity. Notably, the production of TD-Ag-specific antibodies by lung epithelial cells was found to be dependent on CD4+ T cells but not CD8+ T cells. Our findings indicate for the first time that B cells are not the only source of TD-Ag-specific antibodies but are essential for rapid TD-Ag-specific antibody production by non-B cells. This discovery may reveal a new mechanism for the production of specific antibodies.

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