ddPCR Reveals SARS-CoV-2 Variants in Florida Wastewater
Gering, E.; Colbert, J.; Schmedes, S.; Duncan, G.; Lopez, J. V.; Motes, J.; Weiss, J.; Azarian, T.; Tekin, O.; Blanton, J.
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Wastewater was screened for the presence of functionally significant mutations in SARS-CoV-2 associated with emerging variants of concern (VOC) by ddPCR, and results accorded with sequencing of clinical samples from the same region. We propose that PCR-based screening of wastewater can provide a powerful tool for rapid and inexpensive screening of large population segments for VOC-associated mutations and can hone complementary sampling and sequencing of direct (human) test material to track emerging VOC.
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