Single-cell transcriptome lineage tracing of human pancreatic development identifies distinct developmental trajectories of alpha and beta cells
Lin, L.; Zhang, Y.; Qian, W.; Liu, Y.; Zhang, Y.; Lin, F.; Liu, C.; Lu, G.; Song, Y.; Song, J.; Yang, C.; Li, J.
Show abstract
In comparison to mouse, the developmental process of human islets has not been properly elucidated. The advancement of single cell RNA-seq technology enables us to study the properties of alpha and beta cells at single cell resolution. By using mitochondrial genome variants as endogenous lineage-tracing markers, we found that human alpha and beta cells have different lineage features. This finding suggests specific endocrine progenitors for alpha and beta cells, which is different from mouse islet cells. This strategy was also applied to a study of chemically-induced islet cell reprogramming and was used to help identify artemether-induced alpha-to-beta trans-differentiation in human islets. The computational results of this study will inspire future studies to establish, maintain, and expand beta cell-specific progenitors in vitro and in vivo.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Single-cell differential splicing analysis reveals high heterogeneity of liver tumor-infiltrating T cells 93%
- An integrated Asian human SNV and indel benchmark combining multiple sequencing methods 93%
- Generation of Somatic Mitochondrial DNA-Replaced Cells for Mitochondrial Dysfunction Treatment 93%
Similar papers in this journal
- Single cell RNA sequencing of nc886, a non-coding RNA transcribed by RNA polymerase III, with a primer spike-in strategy 93%
- Single-cell alternative polyadenylation analysis reveals mechanistic insights of COVID-19-associated neurological and psychiatric effects 92%
- Polyploidy of semi-cloned embryos generated from parthenogenetic haploid embryonic stem cells 92%
Similar papers in this journal
- Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes 93%
- ScRNA-seq and scATAC-seq reveal that sertoli cell mediate spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia 93%
- Long-read single-cell sequencing reveals expressions of hypermutation clusters of isoforms in human liver cancer cells 93%
Similar papers in this journal
- IReNA: integrated regulatory network analysis of single-cell transcriptomes 95%
- Type 2 diabetes susceptibility gene GRK5 regulates physiological pancreatic β-cell proliferation via phosphorylation of HDAC5 in mice 92%
- The HDAC inhibitor trichostatin A impairs pancreatic β-cell function through an epigenome-wide reprogramming 92%