Impact of regulative noise exposure to enhanced lipid droplet assembly in Saccharomyces cerevisiae: Preliminary results from a laboratory experiment

Lipid Droplet (LD) is a ubiquitous cellular organelle that stores natural lipids as an energy and carbon source. It has emerged as a highly active organelle, engaged in lipid synthesis, protein storage, protein degradation, transportation, and metabolism. It stores natural lipids in the form of triacylglycerols (TAG) and steryl esters. TAGs consider promising biotechnological importance to produce biodiesel; thus, LD is considered a tremendous scientific concern in the modern era. The TAG accumulation is found in various feedstocks, but amongst the microorganisms becomes an evident alternative against animal and plant-derived sources due to economic reasons. Amid microorganisms, the Saccharomyces cerevisiae is a better alternative for industrial utilization but has low production of TAGs. Thus, to enhance the LD concentration, novel research was designed to induce alternate high and low sound frequency at a regular interval on a yeast model organism. The control and treated yeast samples further investigated using biochemical, biophysical, and computational tools to conclude that cells increase lipid droplet production under regulative noise exposure. The results endorsed that noise induces yeast LD yield is significantly higher than control, which could be considered a milestone in the biodiesel industry development and the biodiesel policy. This analysis also helps researchers to understand the novel function of LDs and their regulation in cell metabolism.