Concentration of the cellular material in the nasopharyngeal swabs increases the clinical sensitivity of SARS-CoV2 RT-PCR
Kannian, P.; Mahanathi, P.; Ashwini, V.
Show abstract
Severe acute respiratory syndrome - coronavirus 2 (SARS-CoV2) is detected by a highly sensitive molecular method, reverse transcriptase-polymerase chain reaction (RT-PCR) from nasopharyngeal swab (NPS) samples collected in 2-3ml of viral transport medium (VTM). Unlike body fluids, NPS samples are undermined by high variability in the amount of cells that get suspended into the VTM. Hence, the cell density used for RNA extraction becomes an important analytical variable that contributes to the overall sensitivity of the RT-PCR. In this study, we compared the sensitivity of SARS-CoV2 RT-PCR in 50 NPS samples collected from in-patients of the COVID wards using the concentration and direct methods. The concentration method detected the viral RNA in all 50 samples, while the direct method was positive in only 41 (82%) samples (p=0.003). Additionally, the Ct values were lower in the direct method compared to concentration method among the 41 positive samples (p=0.03 for N gene and p=0.04 for RdRp gene). The mean CV% was also [≥]10%. Thus, the concentration of the cells prior to RNA extraction drastically improves the sensitivity of detection of SARS-CoV2 in NPS samples.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Development and Evaluation of AccuPower® COVID-19 Multiplex Real-Time RT-PCR Kit and AccuPower® SARS-CoV-2 Multiplex Real-Time RT-PCR Kit for SARS-CoV-2 Detection in Sputum, NPS/OPS, Saliva and Pooled Samples 96%
- Evaluation of silver nanoparticles for the prevention of SARS-CoV-2 infection in health workers: in vitro and in vivo 95%
- Validation of saline, PBS and a locally produced VTM at varying storage conditions to detect the SARS-CoV-2 virus by qRT-PCR 94%
Similar papers in this journal
- A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR 95%
- Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis 94%
- The virucidal effects of 405 nm visible light on SARS-CoV-2 and influenza A virus 93%
Similar papers in this journal
Similar papers in this journal
- Analysis Of Four Different Transport And Preservation Medium Kits For SARS-COV-2 Diagnosis From Nasopharyngeal Swab By Real-Time PCR: Adapting To The Constantly Increasing Demand Of Sampling Processing And Stock-Outs During The Pandemic 98%
- Evaluation of seven different rapid methods for nucleic acid detection of SARS-COV-2 virus 96%
- Clinical evaluation of an automated, rapid mariPOC ® antigen test in screening of symptomatics and asymptomatics for SARS-CoV-2 infections 94%
Similar papers in this journal
- Misinterpretation of viral load in COVID-19 96%
- Implications in the quantitation of SARS-CoV2 copies in concurrent nasopharyngeal swabs, whole mouth fluid and respiratory droplets 95%
- Inactivation of SARS-CoV-2 by β-propiolactone Causes Aggregation of Viral Particles and Loss of Antigenic Potential 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.