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SCV-2000bp: a primer panel for SARS-CoV-2 full-genome sequencing

Speranskaya, A.; Kaptelova, V.; Valdokhina, A.; Bulanenko, V.; Samoilov, A.; Korneenko, E.; Shipulina, O.; Akimkin, V.

2020-08-04 molecular biology
10.1101/2020.08.04.234880 bioRxiv
Show abstract

Here we provide technical data for amplifying the complete genome of SARS-CoV-2 from clinical samples using only seventeen pairs of primers. We demonstrate that the [C]V2000bp primer panel successfully produces genomes when used with the residual total RNA extracts from positive clinical samples following diagnostic RT-PCRs (with Ct in the range from 13 to 20). The library preparation method reported here includes genome amplification of ~1750-2000 bp fragments followed by ultrasonic fragmentation combined with the introduction of Illumina compatible adapters. Using the SCV2000bp panel, 25 complete SARS-CoV-2 virus genome sequences were sequenced from clinical samples of COVID-19 patients from Moscow obtained in late March - early April.

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