DNA extraction and sequencing of the Mawangdui ancient cadaver protected by formalin
Huang, H.-L.; Yin, S.; Zhao, H.; Tian, C.; Huang, J.; Deng, S.; Li, Z.
Show abstract
Mawangdui ancient Cadaver is the first wet corpse found in the world, which is famous for being immortal for over two thousands of years. After being unearthed, the female corpse was immersed in the formalin protective solution for more than 40 years. We used magnetic bead method and formalin fixed paraffing (FFPE) method to extract the DNA of the female corpse, respectively. PCR amplification, sanger sequencing, library building, high throughput sequencing (testing) and data processing were carried out on the DNA samples, and about 0.5% of the whole genome coverage sequencing data was obtained. Comparing the results of DNA trough two extraction and sequencing methods. We found that the FFPE and high throughput sequencing methods is better than others for DNA extraction of the ancient samples which were preserved in formalin, providing a guidance for dealing with formalin preserved ancient samples in the future.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Comparative analysis of novel MGISEQ-2000 sequencing platform vs Illumina HiSeq 2500 for whole-genome sequencing 93%
- Ancient DNA analysis of food remains in human dental calculus from the Edo period, Japan 93%
- Mitochondrial DNA is a sensitive surrogate and oxidative stress target in oral cancer cells 93%
Similar papers in this journal
- Non-destructive DNA extraction from specimens and environmental samples using DESS preservation solution for DNA barcoding 94%
- Pre-Sequencing Assessment of RNA-Seq Library Quality Using Real-Time qPCR 93%
- A low-cost platform suitable for sequencing-based recovery of natural variation in understudied plants. 92%
Similar papers in this journal
- Genomic DNA extraction optimization and validation for genome sequencing using the marine gastropod Kellet's whelk 93%
- Ursolic acid improves the bacterial community mapping of the intestinal tract in liver fibrosis mice 90%
- Resumption of spermatogenesis in senescent goldfish Carassius auratus (Linnaeus, 1758) through spermatogonial cell therapy 90%
Similar papers in this journal
- Automated phenol-chloroform extraction of high molecular weight genomic DNA for use in long-read single-molecule sequencing. 92%
- Shark and ray genome size estimation: methodological optimization for inclusive and controllable biodiversity genomics 92%
- Design-of-Experiments In Vitro Transcription Yield Optimization of Self-Amplifying RNA 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.