Elemental analysis of Moringa oleifera seeds by Laser Induced Breakdown Spectroscopy (LIBS) and its anti-cancer and anti-microbial studies

Moringa oleifera plant contains a numerous antioxidants, antibiotics and nutrients (vitamins and minerals) which makes it prospective for diverse biomedical applications. This report investigated the anti-cancerous and anti-microbial potential of the Moringa oleifera seeds (MOSs) due to the bioactive components a detail elemental analysis. MOSs in the form of pellets were used in elemental analysis. Alcoholic extraction of were utilized in anti-cancerous and anti-microbial activity.To clarify the anti-cancerous and anti-microbial potential of the (MOSs) because of the bioactive components. An elemental analysis was conducted using Laser Induced Breakdown Spectroscopy (LIBS). The GC-MS used for the validation of the LIBS outcome. Additionally, the anti-cancerous and anti-microbial activity of the MOSs was evaluated. Herein, the human colorectal carcinoma cells (HCT-116) were treated with the seeds aqueous extracts for 48 h and the cell viability plus the DNA nuclear morphology were measured via the MTT assay and DAPI staining. The cell viability of the normal human embryonic stem cells (HEK-293) was also examined after the treatment. Percentage of cell viability and inhibitory concentration (IC50) of both normal and treated cells were determined.The recorded LIBS signal of the MOSs revealed the existence of elements like Ca, K, Mg, P, S, Fe, Mn, Zn, Na and Se (vital for human health). The results of the MTT assay revealed a profound inhibitory action of the MOSs extracts against the HCT-116 cells growth. On top, such extracts did not affect the HEK-293 cells growth, indicating the specificity of the proposed extracts towards cancer cells escalation hindrance. The anti-microbial activity of the ethanolic MOSs extracts was tested on the S. aureus and E. coli bacteria using the Agar well diffusion assay. The observed anti-cancerous and the anti-microbial activities of the MOSs extracts can be attributed to the charisma of various bioactive compounds including the oleic acid, palmitic acid, hexadecenoic acid ethyl ester and D-allose. ConclusionCurrent observations may contribute towards the development of the MOSs-based biomedicine (organic without any side effects, cheap, plentiful, pure and sustainable) effective for the cancer and bacterial infection cure.