In vetro study on the toxic effects of acrylamide on blood antioxidants, ameliorative effects of vitamin C

BackgroundAcrylamide (ACR) is a naturally occurring, widely used compound, it is generated during cocking carbohydrate rich food at high temperature. Ingestion of large amounts of ACR underlies several health concerns and teratogenicity. Ascorbic acid (vit C) is a strong reducing agent greatly used to clean free radicals. Materials and methodsBlood sample was obtained from 46 years old, healthy nonsmoking man in heparinized tubs. Blood sample was immediately divided into seven parts as triplet for each. The first one was leaved as control, 2nd, 3rd and the 4th were treated with acrylamide in a concentration of 25,50 and 100 mM respectively, the 5th, 6th and the 7th were treated with acrylamide as the mentioned concentrations and vitamin C in a concentration of 100mM. Samples (one mile litter) from each tube were taken after four and 24 hours and were used for preparation of hemolysates, that were kept at -80{degrees}C till investigation of the biochemical parameters. ResultsThe concentrations of Malondialdehyd (MDA), nitric oxide (NO) and hydrogen peroxide (H2O2) increased in ACR and/or vit. C treated samples as compared with control. The concentration of reduced glutathione (GSH) and activities of Catalase (CAT), Superoxide dismutase (SOD), Glutathione reductase (GR), Glutathione peroxidase (GPx) and glucose 6 phosphate dehydrogenase (G6PDH) decreased significantly in ACR and/or vit. C treated samples as compared with control. Meanwhile, The concentrations of MDA, NO and H2O2 decreased in samples treated with both ACR and vit. C as compared with that treated with ACR only. The concentration of GSH and activities CAT, SOD, GR, GPx and G6PDH increased significantly in samples treated with both ACR and vit. C as compared with that treated with ACR only. ConclusionACR produce its toxic effect through its deleterious action on the antioxidant system through induction of pro-oxidants leading to exhausting of antioxidants. Vitamin C has an ameliorative action on the deleterious action exerted by ACR through improving the balance between pro-oxidants and antioxidant.