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Antioxidant, anti-enzymatic, antimicrobial and cytotoxic properties of Euphorbia tirucalli L.

altamimi, m.; Jaradat, N.; Al-Lahham, S.; Al-masri, M.; Bsharat, A.; Alsaleh, R.; Sabobeh, R.

2019-12-17 pharmacology and toxicology
10.1101/2019.12.17.879692 bioRxiv
Show abstract

Medicinal properties of Euphorbia tirucalli L. have been investigated in vitro. Water extract from the plant latex was freeze dried and tested against 3 digestive enzymes and showed IC50 as 39.8{+/-}0.22, 79.43{+/-}0.38 and 316.22{+/-}0.3 for lipase, alpha- glucosidase and alpha- amylase, respectively. These results were incomparable with drugs such as acarbose and orlistat. Antioxidant property showed that the IC50 values of E. tirucalli and Trolox were 79.43{+/-}0.69 and 3.1 {+/-}0.92 respectively. Also, the plant extract exhibited a range of antimicrobial activity against gram-positive and gram-negative bacteria and pathogenic yeast and fungi. Cytotoxicity of the plant extract tested on Caco-2 cells was determined using MTT method. The effect was linear and almost 0.5 mg/ml extract has inhibited 50% of cells relative to the control. Euphorbia tirucalli showed promising activities and is potential source of active ingredient with functional properties.

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