Identification of active deubiquitinases in dendritic cells under unstimulated and activated conditions.
Morgan, H. B.; Villadangos, J. A.; Edgington-Mitchell, L. E.; Mintern, J. D.
Show abstract
Ubiquitination is a post-translational modification with well described roles in the regulation of immune cell function, such as dendritic cells (DCs). DCs are crucial initiators and regulators of T cell-mediated immune responses. In this study, we use activity-based probes and proteomics to systematically profile DUBs in a DC line. Using the lysate-based biotin(bio)-Ubiquitin(Ub)-propargylamide(Prg) probe and proteomics, the activity and abundance of deubiquitinases was measured in unstimulated and CpG-stimulated MutuDCs to simulate responses to bacteria. This analysis was complemented with profiling using the cell-permeable DUB activity-based probe IMP-2373. The combined analysis with bio-Ub-Prg and IMP-2373 provided insights into the dynamics of DUB activity and abundance in DCs following stimulation and identified several DUBs for further study.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- WITHDRAWN: DiDBiT-TMT: A novel method to quantify changes in the proteomic landscape induced by neural plasticity 92%
- Discovering the N-terminal Methylome by Repurposing of Proteomic Datasets 91%
- Quantitative analysis of non-histone lysine methylation sites and lysine demethylases in breast cancer cell lines 91%
Similar papers in this journal
- Fast and accurate bacterial species identification in biological samples using LC-MS/MS mass spectrometry and machine learning 90%
- Improved peptide search for identification of SUMO and sequence-based modifications, in MaxSBM 89%
- Bayesian Confidence Intervals for Multiplexed Proteomics Integrate Ion-Statistics with Peptide Quantification Concordance 89%
Similar papers in this journal
- Spatiotemporal proximity labeling tools to track GlcNAc sugar-modified functional protein hubs during cellular signaling 90%
- Dual-Probe Activity-Based Protein Profiling Reveals Site-Specific Differences in Protein Binding of EGFR-Directed Drugs 90%
- Discovery of Chemical Tools for Polysorbate-Degradative Enzyme Control in Biopharmaceutical Upstream Process via Multi-Omic Profiling of Host Cell Clones 89%
Similar papers in this journal
- A Proteomic Platform to Identify Off-Target Proteins Associated with Therapeutic Modalities that Induce Protein Degradation or Gene Silencing 91%
- Longitudinal Saliva Omics Responses to Immune Perturbation: A Case Study 90%
- Towards middle-up analysis of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by means of HPLC-MS 90%
Similar papers in this journal
- Development and Comparative Evaluation of Endolysosomal Proximity Labeling-based Proteomic Methods in Human iPSC-derived Neurons 91%
- AUTO-SP: automated sample preparation for analyzing proteins and protein modifications 91%
- High-Sensitive Spatial Proteomics for Pancreatic Cancer Progression Analysis 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.