bioRheoFlow A Rheometric Platform for In Situ Biofilm Mechanics Under Controlled Flow Conditions
Ianniello, V.; Geisel, S.; Secchi, E.; Tervoort, T.; Vermant, J.
Show abstract
Biofilm mechanics depend strongly on growth history, hydration, nutrient availability, and interfacial attachment, yet conventional rheology requires harvesting and transfer that can perturb the as-grown structure. Here, we introduce bioRheoFlow, a 3D-printed rheometer-mounted platform for cultivation and mechanical characterization without sample transfer. The platform combines evaporation compensation with static or axial nutrient flow, and benchmark measurements validate its rheometric performance. Applied to Pseudomonas putida and Pantoea sp., bioRheoFlow reveals that biofilms cultivated in situ under axial flow exhibit substantially higher elastic moduli than conventionally grown and scraped samples. Despite similar linear-viscoelastic stress ranges, the species show distinct nonlinear responses: P. putida exhibits a lower crossover stress and more abrupt failure, whereas Pantoea sp. sustains larger stresses and deformations. Creep suggests contributions from bulk deformation and biofilm-substrate interactions. bioRheoFlow provides a controlled framework for probing growth-conditioned mechanics in living biofilms.
Matching journals
The top 13 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A fatigue-resistant myoneural actuator for implantable biohybrid systems 92%
- A hybrid additive manufacturing platform to create bulk and surface composition gradients on scaffolds for tissue regeneration 92%
- Bacterial cellulose spheroids as building blocks for 2D and 3D engineered living materials 92%
Similar papers in this journal
- Live imaging of Aiptasia larvae, a model system for studying coral bleaching, using a simple microfluidic device 94%
- Polyacrylamide Bead Sensors for in vivo Quantification of Cell-Scale Stress in Zebrafish Development 93%
- Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections. 92%
Similar papers in this journal
- High-throughput viscoelastic characterization of cells in hyperbolic microchannels 93%
- You will know by its tail: a method for quantification of heterogeneity of bacterial populations using single cell MIC profiling 92%
- Microfabrication-based engineering of biomimetic dentin-like constructs to simulate dental aging 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.