Defining the spectrum of cellular protection against cadmium stress by Dsup expressed in yeast
Hitchens, L.; Gaikani, H. K.; Barazandeh, M.; Giaever, G.; Nislow, C.
Show abstract
The intrinsically disordered tardigrade Dsup protein has garnered attention for its ability to protect cells and organisms from radiation and oxidative stress. Our lab has previously described Dsups protective effect in yeast, finding that, while Dsup expression comes with a fitness cost, it improves survival under many stressors, including the heavy metal cadmium. While Dsups protection against oxidative stress and radiation is well-established, the mechanism by which it protects against other stressors is underinvestigated. To address this knowledge gap, we screened a collection of 4000 yeast deletion mutants expressing Dsup in the presence of three doses of cadmium to identify synergistic/synthetically lethal genes and processes. We find that Dsup broadly buffers many processes/genes at lower levels of cadmium stress but at higher doses, cells shift to buffering specific genes/processes. These results identify host genetic processes that influence Dsups ability to confer cadmium resistance to organisms.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Temperature preference biases parental genome retention during hybrid evolution 94%
- Mediator subunit MDT-15/MED15 and Nuclear Receptor HIZR-1/HNF4 cooperate to regulate toxic metal stress responses in Caenorhabditis elegans 93%
- Joint effects of genes underlying a temperature specialization tradeoff in yeast 93%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.