A meiotic B-type cyclin selectively drives mitotic progression and fidelity in cancer cells
Kizhedathu, A.; Nguyen, A. C. N.; Yu, C.; Moghareh, S.; Tran, V.; Mousaian, P.; Vertiz, J.; Benavente, C. A.; Huang, L.; Lara-Gonzalez, P.
Show abstract
Cell cycle transitions are driven by cyclins in complex with cyclin-dependent kinases (CDK). Here, we demonstrate that cyclin B3, an evolutionarily divergent B-type cyclin previously thought to function exclusively in female meiosis, is expressed in mitotic cells and interacts with both CDK1 and CDK2. We show that cyclin B3-CDK1/2 is essential for timely mitotic progression by ensuring proper chromosome alignment and stable kinetochore-microtubule interactions, while its overexpression accelerates mitosis and improves chromosome alignment kinetics. Through phospho-mass spectrometry, we identify several kinetochore and mitotic spindle components as substrates of cyclin B3-CDK1/2 in mitosis. Notably, cyclin B3 depletion disrupts mitotic progression in most cancer cell lines tested - including colorectal, osteosarcoma, and breast cancer models - while non-transformed cells are largely unaffected. These findings identify cyclin B3-CDK1/2 as a novel driver of mitotic progression and reveal a preferential dependency in a subset of cancer cells that may represent a potential therapeutic vulnerability.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Replication stress induces mitotic death through parallel pathways regulated by WAPL and telomere deprotection 94%
- Polycomb Repressive-Deubiquitinase Complex Safeguards Oocyte Epigenome and Female Fertility by Restraining Polycomb Activity 94%
- Proper chromosome alignment depends on BRCA2 phosphorylation by PLK1 94%
Similar papers in this journal
Similar papers in this journal
- CDK1-dependent N-terminal NuMA phosphorylation promotes dynein-dynactin-NuMA assembly for accurate chromosome segregation 95%
- Cyclin A and Cks1 promote kinase consensus switching to non-proline directed CDK1 phosphorylation 94%
- An Aurora kinase A-BOD1L1-PP2A B56 Axis promotes chromosome segregation fidelity 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.