Integrative Nanopore and Illumina sequencing reveals age-associated tRNA modification and CCA-tail dynamics in yeast
Brazell, K. M.; Prevost, C.; Zawrotna, N.; Smith, C. K.; Power, L. N.; Smith, J. S.; Su, Z.
Show abstract
Aging is characterized by a progressive loss of proteostasis. Transfer RNAs (tRNAs) are essential regulators of translation, yet their dynamics during aging remain poorly understood due to challenges in sequencing highly modified RNAs. Here we present a benchmarked Nanopore direct RNA sequencing (RNA004 chemistry) resource that profiles the Saccharomyces cerevisiae tRNAome during replicative aging at single-molecule resolution. Using in vitro transcribed tRNA controls, we establish modification detection thresholds and validate key findings with orthogonal Illumina sequencing. While overall tRNA abundance remains largely stable, our resource reveals age-associated terminal A cleavage at the 3' CCA tail of mature tRNAs, targeted T-loop and anticodon modification changes, and single-molecule evidence of modification co-occurrence. This dataset provides a resource for exploring tRNA regulation, translation fidelity, and longevity.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The trypanosome mRNA decapping enzyme ALPH1 prefers caps without m7G methylation and produces diphosphate-RNA 92%
- Role of Era in Assembly and Homeostasis of the Ribosomal Small Subunit 92%
- Replicative aging is associated with loss of genetic heterogeneity from extrachromosomal circular DNA in Saccharomyces cerevisiae 92%
Similar papers in this journal
- Aging-associated decline of phosphatidylcholine synthesis is a malleable trigger of natural mitochondrial aging. 94%
- Redefining human pre-rRNA processing at single nucleotide resolution using long read Nanopore sequencing 94%
- Precision Estimates of Longitudinal Brain Aging Capture Unexpected Individual Differences in One Year 93%
Similar papers in this journal
- Allele-specific splicing modulates protein isoforms and Alzheimer's risk 92%
- Quantitative mitochondrial DNA copy number determination using droplet digital PCR with single cell resolution: a focus on aging and cancer 92%
- A murine aging cell atlas reveals cell identity and tissue-specific trajectories of aging 92%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.