Diversity of penicillin-binding protein 2x in Streptococcus pyogenes from England and Wales, 2001 to 2015
Sveikauskaite, G.; Marshall, A. L.; Gorshkova, S.; Guy, R. L.; Wan, Y.; Vieira, A.; Coelho, J.; Moganeradj, K.; Sriskandan, S.; Jauneikaite, E.; Soo, V. W.
Show abstract
Streptococcus pyogenes remains clinically susceptible to {beta}-lactam antibiotics, its first-line treatment. Yet recent reports from the US, Iceland, and Japan have identified isolates with PBP2x (a primary {beta}-lactam target) substitutions that reduced antibiotic susceptibility. To determine whether these PBP2x substitutions occurred in S. pyogenes from England and Wales, we re-analysed 2,970 invasive genomes from 2001 to 2015. We found 42 PBP2x sequence mutants in 34% (996/2,970) of the genomes, revealing an unexpected diversity in this antibiotic target. Nine emm12 isolates carried a novel double substitution, PBP2x G600A_P601H, that co-occurred with macrolide resistance genes. Experimentally, these isolates showed 2- to 4-fold elevated minimum inhibitory concentrations for four {beta}-lactam antibiotics, alongside erythromycin resistance. Other PBP2x substitutions, found across seven emm types without reduced antibiotic susceptibility, appeared driven by emm lineage expansion rather than antibiotic pressure. These findings establish a baseline for pbp2x variation, reinforcing the case for sustained genomic surveillance globally.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The global phylogenetic landscape and nosocomial spread of the multidrug-resistant opportunist Stenotrophomonas maltophilia 95%
- Genomic dissection of the bacterial population underlying Klebsiella pneumoniae infections in hospital patients: insights into an opportunistic pathogen 95%
- Increased antibiotic susceptibility in Neisseria gonorrhoeae through adaptation to the cervical environment 94%
Similar papers in this journal
Similar papers in this journal
- Piperacillin/tazobactam resistant, cephalosporin susceptible Escherichia coli bloodstream infections are driven by multiple acquisition of resistance across diverse sequence types 94%
- Context-aware genomic surveillance reveals hidden transmission of a carbapenemase-producing Klebsiella pneumoniae 94%
- The clinical, genomic, and microbiological profile of invasive multi-drug resistant Escherichia coli in a major teaching hospital in the United Kingdom 94%
Similar papers in this journal
Similar papers in this journal
- Molecular epidemiology of Escherichia coli and Klebsiella species bloodstream infections in Oxfordshire (UK) 2008-2018 94%
- Longitudinal analysis within one hospital in sub-Saharan Africa over 20 years reveals repeated replacements of dominant clones of Klebsiella pneumoniae and stresses the importance to include temporal patterns for vaccine design considerations 94%
- ESBL plasmids in Klebsiella pneumoniae : diversity, transmission, and contribution to infection burden in the hospital setting 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.