Genome-wide overexpression screen of Pseudomonas putida phage Emajogi
Diaz, B.; Won, S.; Padala, M.; Mageeney, C. M.
Show abstract
Bacteriophage genomes contain large fractions of uncharacterized genes, motivating high-throughput functional screens to illuminate their roles in host interaction and cytotoxicity. Here, we performed a genome wide overexpression assay for a Pseudomonas putida KT2440 infecting phage, Emajogi, and identified eight cytotoxic genes that reduce host growth under both basal and induced expression. We show that these proteins are conserved across phages that infect a diverse set of hosts. The most cytotoxic gene identified was a hypothetical protein (gp18) with no predicted functional domains. Transcriptomic profiling revealed that overexpression of gp18 caused a pronounced metabolic remodeling of the cell, likely shifting cell resources to phage production. Overall, this genome wide screen provides information about phage genes that were previously unannotated that can support biotechnology and novel antimicrobial development.
Matching journals
The top 11 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Structural investigation of QatB and QatC proteins in QatABCD anti-phage defense 94%
- Prevalent Gut Phages Encode Modular Adhesins Mediating Epithelial Binding and Endoplasmic Reticulum Trafficking 94%
- Strengthening Phage Resistance of Streptococcus thermophilus by Leveraging Complementary Defense Systems 94%
Similar papers in this journal
- Culture- and genome-based characterization of a tripartite interaction between patescibacterial epibionts, methylotrophic proteobacteria, and a jumbo phage in freshwater ecosystems 93%
- Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract 93%
- Aminoglycoside antibiotics inhibit phage infection by blocking an early step of the phage infection cycle 93%
Similar papers in this journal
- Mycobacterium phage Butters-encoded proteins contribute to host defense against viral attack 93%
- Species-scale genomic analysis of S. aureus genes influencing phage host range and their relationships to virulence and antibiotic resistance genes 93%
- Proteomic and transcriptomic analysis of Microviridae {varphi}X174 infection reveals broad up-regulation of host membrane damage and heat shock responses 92%
Similar papers in this journal
- Bacteriophages target membrane-anchored glycopolymers to promote host cell lysis and progeny release 95%
- Definitive demonstration by synthesis of genome annotation completeness 93%
- FliO is an evolutionarily conserved yet diversified core component of the bacterial flagellar type III secretion system 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.