Fully Two-Photon-Driven RESOLFT Microscopy for Super-Resolution Imaging inside Tissue
Ozaki-Noma, R.; Ishii, H.; Wazawa, T.; Sakamoto, J.; Kozawa, Y.; Kato, Y.; Mukai, E.; Nemoto, T.; Nagai, T.
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Two-photon excitation (2PE) microscopy reaches deep into tissue but remains diffraction-limited, and existing 2PE-STED and 2PE-RESOLFT schemes still rely on single-photon depletion or photoswitching. Here we present 2P-RESOLFT microscopy, in which excitation and reversible photoswitching are both two-photon-driven. Using the positive photoswitchable protein Padron2, we achieved 205-nm lateral resolution and resolved previously unreported membrane protrusions of {beta} cells inside pancreatic pseudoislets.
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