A pyoverdine-type metallophore is required for lanthanide-dependent growth in Pseudomonas putida
Rasmussen, K.; Michael, J.; Benson, A. F.; Ramirez, K. J.; Ingraham, M. A.; Aron, A. T.; Werner, A. Z.
Show abstract
Lanthanides (Ln) are recently discovered cofactors for alcohol metabolism in a growing number of bacteria, including the soil-dwelling bacterium Pseudomonas putida, yet the mechanisms for Ln uptake are poorly defined. Here, we discovered that the non-ribosomal peptide synthase biosynthetic gene cluster, pvdLIJD, was essential to Ln-dependent growth in P. putida. Pyoverdine G4R and its precursor ferribactin were identified as pvdLIJD gene products with La-responsive abundances, and both chelated La as well as iron. Deletion of the putative pyoverdine outer membrane transport system encoded by fpvA and exbBD:tonB were strongly linked to Ln-dependent growth in a concentration-dependent manner. Global transcriptomes were remodeled in response to La, but weak evidence of transcriptional pvdLIJD regulation and no evidence of discernment between La provided as chloride versus oxide were observed. Overall, this work establishes pyoverdines as metallophores involved in La uptake, re-framing them from strictly iron-scavenging siderophores to dual-purpose metallophores with Ln-binding activity.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Integrated cellular and molecular responses to uranium chemotoxicity in the metal-tolerant microalga Coelastrella sp. PCV 94%
- Classification of the glyphosate target enzyme (5-enolpyruvylshikimate-3-phosphate synthase) 92%
- Identification of uranyl-binding proteins in Arabidopsis thaliana cells exposed to uranium: Insights from a metalloproteomic analysis and characterization of Glycine-Rich RNA-binding Protein 7 (GRP7) 90%
Similar papers in this journal
- A novel and evolutionarily distinct flavoprotein monooxygenase drives skatole degradation in Rhodococcus 94%
- Identification of a Highly Expressed Gene Cluster Likely Coding for Benzene Activation Enzymes in a Methanogenic Enrichment Culture 93%
- Burkholderia Genes Involved in Extracellular Bioplastic Degradation Revealed by Tn-seq and CRISPR-Cas 93%
Similar papers in this journal
- Revisiting oxygen toxicity: evolution and adaptation to superoxide in a SOD-deficient bacterial pathogen 93%
- Metabolic exchange and energetic coupling between nutritionally stressed bacterial species, the possible role of QS molecules 92%
- How thermophilic Gram-positive organisms perform extracellular electron transfer: characterization of the cell surface terminal reductase OcwA 92%
Similar papers in this journal
- The Iron Metalloproteome of Pseudomonas aeruginosa Under Oxic and Anoxic Conditions 92%
- Metalation calculators for E. coli strain JM109 (DE3): Aerobic, anaerobic and hydrogen peroxide exposed cells cultured in LB media 92%
- WITHDRAWN: Nitrogen sources enhance siderophore-mediated competition for iron between potato common scab and late blight causative agents 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.