Paired-surface spatial mechanomics links tissue stiffness maps to spatial transcriptomics
Ong, H. T.; Lou, Y.; Turley, J.; Hengst, R. M.; Ramli, M. F. H.; Shen, X.; Marlena, J.; Zhu, J.; Li, R.; Chan, C. J.; Young, J. L.
Show abstract
Tissue mechanics influence diverse biological processes, yet directly linking stiffness measurements to spatially resolved molecular states in intact tissues remains challenging. Here we developed a paired-surface spatial mechanomics approach to map Young's modulus by nanoindentation on a fresh tissue surface and co-register the stiffness grid with 10x Genomics Visium HD spatial transcriptome bins from the immediately adjacent, parallel surface. Applied to the mouse ovary, which has spatially distinct compartments and undergoes extracellular matrix remodeling with cycle and age, the workflow generated >2,900 matched measurements across 21 regions of interest. Nanoindentation at 50-m grid spacing enabled millimeter-scale stiffness maps while balancing acquisition time in fresh tissues, with ~92 4-m transcriptome bins assigned to each stiffness value. Global and compartment-specific analyses associated stiffer regions with lower elastic fiber programs and higher inflammatory signaling, with age-dependent differences. This correlative strategy integrates experimentally measured mechanics with spatial omics in fresh tissues.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Biomechanical Phenotyping Reveals Unique Mechanobiological Signatures of Early-Onset Colorectal Cancer 92%
- Material-mediated histogenesis using mechano-chemically microstructured cell niches 92%
- Single-cell metabolic imaging and digital scoring of fat tissue remodeling by label-free metabolic imaging 92%
Similar papers in this journal
- Light-sheet photonic force optical coherence elastography for high-throughput quantitative 3D micromechanical imaging 93%
- Temporo-spatial cellular atlas of the regenerating alveolar niche in idiopathic pulmonary fibrosis 93%
- Modular actin nano-architecture enables podosome protrusion and mechanosensing 93%
Similar papers in this journal
- Hybrid hydrogel-extracellular matrix scaffolds identify distinct ligand and mechanical signatures in cardiac aging 93%
- Unjamming overcomes kinetic and proliferation arrest in terminally differentiated cells and promotes collective motility of carcinoma. 92%
- The laminin-keratin link shields the nucleus from mechanical deformation and signalling 92%
Similar papers in this journal
- Three-dimensional tissue stiffness mapping in the mouse embryo supports durotaxis during early limb bud morphogenesis 93%
- Mechanophenotyping of 3D Multicellular Clusters using Displacement Arrays of Rendered Tractions 93%
- Immune cells employ traction forces to overcome steric hindrance in 3D biopolymer networks 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.