Development of iPSC-derived urothelial organoids towards investigating the effect of hormones on host-defense to urinary tract infections
Bindas, A.; Fang, Z.; Boekhorst, J.; Fernandes, A. M.; Wells, J.
Show abstract
Recurrent urinary tract infection represents a substantial unmet public health in women. Local administration of estradiol has been shown to reduce recurrence, however in vitro models of the female urinary tract remain limited and the mechanisms underlying the effects of estradiol are incompletely understood. Here, we describe a novel iPSC organoid differentiation protocol and its application to establish a multilayered transwell barrier culture model. Estradiol treatment resulted in reduced expression of innate antimicrobial peptides and cytokines, together with increased expression of demannosylation pathways. Treatment of transwell cultures with a combination of female sex hormones reduced endogenous CXCL8 signaling, independently of a 24-hour uropathogenic Escherichia coli (UPEC) challenge. To our knowledge, this is the first iPSC organoid-derived model of the urinary tract, which provides a platform for investigating interactions between the urothelium, urobiome and hormonal environment.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Targeted gene expression profiling for accurate endometrial receptivity testing 92%
- Myofascial Frequency Syndrome: A novel syndrome of bothersome lower urinary tract symptoms associated with myofascial pelvic floor dysfunction 92%
- Mechanical Properties of Native and Decellularized Reproductive Tissues: Insights for Tissue Engineering Strategies 91%
Similar papers in this journal
- Transcriptional profiling of mucus production and modification in rhesus macaque endocervical cells under hormonal regulation 93%
- Establishment and Characterization of a New Immortalized Human Adenomyosis Epithelial-Like Cell Line, tAEC21 92%
- Increased FOXL2 Expression Alters Uterine Structures and Functions 92%
Similar papers in this journal
- Evaluation of the efficacy of Lactobacillus -containing feminine hygiene products on vaginal microbiome and genitourinary symptoms in pre- and postmenopausal women: A pilot randomized controlled trial 93%
- Uterine secretome initiates growth of gynecologic tissues in ectopic locations. 92%
- Conditional deletion of Tmem63b does not impact mouse voiding behavior 92%
Similar papers in this journal
- miRNA/mRNA analysis of increased TGF-β pathways drive epithelial-mesenchymal transition and regulatory T cell differentiation 93%
- Urinary Antimicrobial Peptides and Cytokines as Biomarkers for Recurrent Urinary Tract Infection in Children and Adolescents 93%
- Cervical gene delivery of the antimicrobial peptide, Human β-defensin (HBD)-3, in a mouse model of ascending infection-related preterm birth 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.