Thermodynamic properties and stability of HMGB1 complexes with linear polyelectrolytes elucidated by nano differential scanning fluorimetry
Watson, J.; Klumpp, A.; Kagelmacher, M.; Moon, E.; Traviankina, M.; Krage, C.; Pigaleva, M.
Show abstract
The High Mobility Group Box 1 (HMGB1) protein performs multiple essential functions in the body, ranging from DNA regulation to the activation and mediation of immune responses. However, HMGB1 has been also implicated in several pathological conditions, such as rheumatoid arthritis, sepsis, autoimmune diseases, tumors, and Alzheimer's disease. As a result, HMGB1 is of increasing interest as a therapeutic target. Binding to heparin has been reported to inhibit HMGB1's pathological activity during sepsis in clinical settings. In this work, we compare the interactions of HMGB1 with heparin and its' synthetic analog linear polyglycerol sulfate (lPGS) from the viewpoint of stability and changes to association behavior. This analysis focuses on thermal stability, secondary-structure changes, and particle-size evolution using nano-differential scanning fluorimetry (nanoDSF), circular dichroism spectroscopy (CD), and dynamic light scattering (DLS).
Matching journals
The top 11 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Fabrication of Molecular Blocks with High Responsiveness to the Cancer Microenvironment by Ursodeoxycholic Acid 91%
- Protometabolically generated NADH mediates material properties of aqueous dispersions to coacervate microdroplets 90%
- Natural and engineered isoforms of the inflammasome adaptor ASC form non-covalent, pH-responsive hydrogels 90%
Similar papers in this journal
- Allosteric Residue Dynamics in Insulin: Temperature Induces Shift from Dihexamer to Hexamer Collective Motion 93%
- Multivalent interaction induces phase separation and formation of more toxic aggregates of α-syn in a yeast model of Parkinson's disease 92%
- Convergent behavior of extended stalk regions from staphylococcal surface proteins with widely divergent sequence patterns 91%
Similar papers in this journal
Similar papers in this journal
- Comparative analysis of drug-salt-polymer interactions by experiment and molecular simulation improves biopharmaceutical performance 93%
- NMR-based analysis of nucleotide π-stacking in a crowded environment: Implications for prebiotic reactions 91%
- Transient Formation of Supramolecular Complexes Between Hyaluronan and Oligopeptides at Submicromolar Concentration 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.