Endogenous gene tagging in the model brown alga Ectocarpus using a simplified CRISPR/Cas method
Paix, A.; Raphalen, M.; Avdievich, E.; Agullo, F.; Luthringer, R.; Coelho, S. M.
Show abstract
Brown algae represent one of the few eukaryotic lineages to have independently evolved complex multicellularity, providing a powerful comparative system for investigating the molecular and evolutionary principles underlying multicellular development. Ectocarpus has emerged as the principal model for this lineage, supported by extensive genomic and transcriptomic resources. However, mechanistic and functional studies have remained limited by the available reverse-genetic tools. While recent CRISPR-Cas developments have enabled targeted gene knock-outs, the lack of knock-in (KI) approaches for endogenous protein tagging and precise genomic insertion remains a major experimental bottleneck. Here, we establish a comprehensive CRISPR-Cas genome-engineering framework for Ectocarpus that enables both targeted gene disruption and precise genomic insertion. We demonstrate efficient knock-in of multiple peptide tags at endogenous loci, enabling direct analysis of native proteins. By combining robust gene knock-out with endogenous protein tagging, this framework substantially expands the experimental possibilities for brown algal research and establishes Ectocarpus as a genetically tractable system for functional genomics, providing a foundation for genome engineering across stramenopiles.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Comparative transcriptomics reveals the molecular toolkit used by an algivorous protist for cell wall perforation 92%
- The expanded Bostrychia moritziana genome unveils evolution in the most diverse and complex order of red algae 91%
- Hijacking and Integration of Algal Plastids andMitochondria in a Polar Planktonic Host 91%
Similar papers in this journal
Similar papers in this journal
- Golden magic: RSH enzymes for (p)ppGpp metabolism in the diatom Phaeodactylum tricornutum. 92%
- Gene knockdown via electroporation of short hairpin RNAs in embryos of the marine hydroid Hydractinia symbiolongicarpus 92%
- Transfer of the synechan biosynthesis and regulatory pathway enables sulfated polysaccharide production in Synechococcus elongatus PCC 7942 91%
Similar papers in this journal
- Designer Sinorhizobium meliloti strains and multi-functional vectors for direct inter-kingdom transfer of high G+C content DNA 93%
- Identification of large-scale genomic rearrangements during wheat evolution and the underlying mechanisms 90%
- gUMI-BEAR, a modular, unsupervised population barcoding method to track variants and evolution at high resolution 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.