Spatio-temporally regulated maternal RNA decay factor essential for zebrafish development
Kushawah, G.; Aldas Bulos, V. D.; Baia Amaral, D.; Hassan, H.; Nowotarski, S. H.; Bazzini, A. A.
Show abstract
Maternal mRNA decay is essential for the Maternal-to-Zygotic Transition (MZT), yet its impact on spatial regulation has remained largely unexplored. We identify Cth1 as the first maternally encoded RNA-decay factor regulated in a spatiotemporal manner outside the germline in zebrafish, driven by conserved 3'UTR cis-elements. RfxCas13-dmediated depletion of maternal cth1 revealed early developmental defects that could not be assessed with SpyCas9 mutants due to infertility from impaired gametogenesis. Mechanistically, Cth1 acts independently of zygotic genome activation, recognizing AUrich motifs in maternal 3'UTRs to promote deadenylation and decay in a spatio-temporal manner; deletion of these motifs or depletion of Cth1 stabilizes both reporter and endogenous transcripts. These findings establish Cth1 as a key component of the maternal program and provide the first direct evidence that spatio-temporally regulated mRNA decay, outside the germline, contributes to early vertebrate development.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- tRNA-derived RNA processing in sperm transmits non-genetically inherited phenotypes to offspring in C. elegans 96%
- Polycomb Repressive-Deubiquitinase Complex Safeguards Oocyte Epigenome and Female Fertility by Restraining Polycomb Activity 96%
- MRE11-RAD50-NBS1 activates Fanconi Anemia R-loop suppression at transcription-replication conflicts 95%
Similar papers in this journal
- The SPN-4 Rbfox RNA-binding protein selects maternal mRNAs for CCR4-NOT-dependent clearance in early Caenorhabditis elegans embryos 96%
- DUX is a non-essential synchronizer of zygotic genome activation 95%
- A conserved C. elegans zinc finger-homeodomain protein, ZFH-2, continuously required for structural integrity and function of alimentary tract and gonad 94%
Similar papers in this journal
- Nuclear RNA export factor variant initiates piRNA-guided co-transcriptional silencing 96%
- Precursor miRNAs are trafficked along axons associated with vesicles and locally processed to regulate growth cone steering 95%
- Arginine methylation of DDX5 RGG/RG motif by PRMT5 regulates RNA/DNA resolution. 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.