Disruption of sRNA Function Using Synthetic Arginine Rich Motif Peptides
Ortiz, E. E.; Batresian, A. J.; Punzalan, J. D.; Gutierrez Garcia, A.; Bjornsson, B.; Khoroz, I.; Abrol, R.; Takahashi, M. K.
Show abstract
Small RNAs (sRNAs) regulate the expression of many genes including those involved in antibiotic resistance and bacterial virulence, making them potential therapeutic targets. A molecule that binds an sRNA could interfere with its ability to bind its target mRNA and disrupt the regulation mechanism. Randomization and screening of natural arginine rich motif (ARM) peptides led to peptides capable of interfering with the sRNA MicF's ability to regulate ompF in Escherichia coli. Molecular dynamics simulations suggested that this effect was not a result of a direct disruption of the MicF-ompF interaction. Instead, the peptides interfere with binding of the chaperone Hfq, which is required for MicF-mediated regulation. Subsequent testing demonstrated peptide specificity for MicF over two other Hfq scaffolds and the ability to disrupt regulation of two additional MicF targets. Together, these findings support the use of synthetic ARMs as a potential tool for modulating sRNA function in bacteria.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Structure and thermodynamics of a UGG motif interacting with Ba2+ and other metal ions: accommodating changes in the RNA structure and the presence of a G(syn)-G(syn) pair 92%
- High-throughput experimental validation of novel hairpin ribozymes 92%
- Biophysical characterizations of the recognition of the AAUAAA polyadenylation signal 92%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- Automated design of highly diverse riboswitches 92%
- Identifying antisense oligonucleotides to disrupt small RNA regulated antibiotic resistance via a cell-free transcription-translation platform 91%
- DNA Structure Design Is Improved Using an Artificially Expanded Alphabet of Base Pairs Including Loop and Mismatch Thermodynamic Parameters 91%
Similar papers in this journal
- Nanomechanics and co-transcriptional folding of Spinach and Mango 93%
- Small Molecule Targeting IRES Domain Inhibits Enterovirus 71 Replication via an Allosteric Mechanism that Stabilizes a Ternary Complex 93%
- Structural insights into context-specific inhibition of bacterial translation by macrolides 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.