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Exercise-mediated biomarker signatures from a combined aerobic and strength training intervention in Singaporean breast cancer patients: findings from the BREXINT Pilot Study

Sitjar, P. H. S.; Periasamy, P.; Tan, S. Y.; Wong, M.; Kukumberg, M.; Adam, S.; Yeong, J. P. S.; Lim, E. H.; Goh, J.

2026-08-18 oncology
10.64898/2026.08.17.26360564 medRxiv
Show abstract

Biomarkers perturbed by exercise-mediated molecular mechanisms, in women with early-stage (stage I-III, non-metastatic) breast cancer are poorly defined, and especially in under-represented Asian cohorts. In this exploratory Breast Cancer Exercise Intervention (BREXINT) pilot study, 15 Asian women were randomized to a combined aerobic and resistance exercise intervention program (n=8) and a control group (n=7). Fasting blood sampling was performed at baseline, 8,16, and 24-week timepoints. Blood parameters were imputed, transformed and screened for intervention-specific variations using IQR-trimmed, paired Wilcoxon tests. Twenty-one blood parameters were found to meet a differential change rule (significance observed in 1 group but not the other). Exercise-associated signatures displayed hematological and cytokine remodeling at 16-weeks. Control-associated signatures include adipokine and renal markers at 16 and 24-weeks. Of note, exercise-driven decrease of IL-10 at 16-weeks (p=0.022) retained significance following linear mixed effects confirmation among screened candidates. IL-10-centred modulation is the most convergent exercise-associated blood derived signature but warrants further validation in larger exercise oncology trials.

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