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Neural Signatures of Conscious Experience During Sleep: A Serial Awakening Study Using High-Density EEG

Selte, A.; Haworth, S. E.; Vannasse, T. J.; Alauddin, T.; Gjini, K.; Philibert-Rosas, S.; Brace, C.; Sevak, B.; Riedner, B.; Kalkach-Aparicio, M.; Tononi, G.; Boly, M.; Struck, A. F.

2026-08-20 neuroscience
10.64898/2026.08.16.745040 bioRxiv
Show abstract

Identifying neural signatures of consciousness remains a central challenge in neuroscience. Sleep offers a tractable model for comparing brain activity in the presence or absence of subjective experience while minimizing behavioral responsiveness confounds. Using overnight 256 electrode high-density EEG in 140 participants and a serial-awakening paradigm, we analyzed 699 non-rapid eye movement (NREM) sleep stage 2 and 3 awakenings (351 dreaming experience, 348 no experience). Features from the 60s preceding awakening included regional spectral power, lagged-coherence connectivity, graph-theoretic metrics and gamma-to-alpha power ratios. Dreaming experiences were associated with shifts in posterior spectral balance, particularly reduced alpha and delta power and increased gamma-related measures, together with altered large scale network organization. In participant-level cross-validated machine-learning analyses, all classifiers performed above chance, with the best ensemble model reaching an ROC-AUC of 0.80 and average precision of 0.80. These findings identify reproducible posterior electrophysiological and network-level signatures of conscious states during NREM sleep.

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