The centromere localization domain of kinetoplastid kinetochore protein KKT2 recognizes the free N-terminus of histone H3
Ciszek, A.; Ludzia, P.; Marciano, G.; Allen, W.; Ishii, M.; Forsyth, S.; Wood, C. W.; Redfield, C.; Akiyoshi, B.
Show abstract
Kinetochores are multiprotein complexes that drive chromosome segregation in eukaryotes. Kinetoplastids, a group of early-diverging eukaryotes that lack canonical kinetochore components, have a unique set of kinetochore proteins. How their kinetochores are assembled specifically at centromeres in the absence of a centromere-specific histone H3 variant CENP-A remains unknown. Here, we demonstrate that the centromere localization (CL) domain of KKT2 has similarities to a ZZ domain. An invariant aspartate present in histone H3-binding ZZ domains is conserved in the KKT2 CL domain. Using nuclear magnetic resonance (NMR) spectroscopy and isothermal titration calorimetry (ITC), we show that Trypanosoma brucei KKT2 CL binds the N-terminus of histone H3. Strikingly, even mono-methylation of the N-terminal amino group of histone H3 abolishes the binding. Our study raises a possibility that centromere-specific kinetochore assembly in T. brucei is ensured by abundant N-terminal methylations of histone H3 in non-centromeric regions.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Two distinct SWI/SNF complexes direct chromatin-linked transcriptional programs in Toxoplasma 95%
- Two structurally mobile regions control the conformation and function of metamorphic meiotic HORMAD proteins 95%
- A divergent Plasmodium NEK4 acts as a key regulator driving the early events of meiosis 94%
Similar papers in this journal
- Dynamic localization of the chromosomal passenger complex is controlled by the orphan kinesins KIN-A and KIN-B in the kinetoplastid parasite Trypanosoma brucei 94%
- Cryo-EM of dynein microtubule-binding domains shows how an axonemal dynein distorts the microtubule 94%
- Mitotic exit is controlled during anaphase by an Aurora B-Cyclin B1/Cdk1 crosstalk 94%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- Simultaneous regulation of cytokinetic furrow and nucleus positions by cortical tension contributes to proper DNA segregation during late mitosis 94%
- Multi-site phosphorylation of yeast Mif2/CENP-C promotes inner kinetochore assembly 93%
- PKN is a sex- and species-specific fertilization factor in brown algae 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.