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Oxytocin reshapes fear control: intensity-dependent prefrontal dominance and whole-brain integration during naturalistic viewing

Fu, K.; Xu, S.; Liu, D.; Zhang, Z.; Liu, Q.; He, J.; Xu, T.; Liu, C.; Wang, J.; Zhang, Y.; Zhou, F.; Zhang, X.; Lan, C.; Han, M.; Li, M.; Liang, Z.; Biswal, B.; Kendrick, K. M.; Zhao, W.; Yao, D.; Becker, B.

2026-08-12 psychiatry and clinical psychology
10.64898/2026.08.11.26360155 medRxiv
Show abstract

Anxiety and maladaptive fear remain difficult to treat, and despite initial promising findings, evidence regarding the anxiolytic and translational potential of oxytocin (OT) remains inconsistent. In a preregistered, randomized, double-blind, placebo-controlled, parallel-group pharmaco-fMRI study in 67 healthy men, we tested whether intranasal OT reduces post-exposure subjective fear during prolonged naturalistic viewing a horror movie comprising independently defined low-, medium-, and high-fear segments. OT reduced subjective fear following naturalistic threat exposure after accounting for pre-exposure baseline ratings. Neuroimaging analyses revealed that OT attenuated recruitment of the dorsolateral prefrontal cortex particularly during higher fear, and enhanced coupling of this region with the bilateral amygdala. At the large-scale network level, OT increased communication between frontoparietal/default-mode control networks and subcortical/limbic networks during high fear indicating more integrative fear regulation. A whole-brain fear neuromarker (CAFE) further confirmed intensity-dependent OT effects. Together, these findings indicate that OT modulates post-exposure fear experience and fear-related neural dynamics in ecologically valid contexts.

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