DJ-1/PARK7 Determines miRNA Network Plasticity During Genotoxic Stress

PARK7 (DJ-1) is a redox-sensitive stress-response protein that supports cellular adaptation, but its role in post-transcriptional responses to genotoxic stress remains unclear. We investigated whether DJ-1 abundance determines the miRNA response to X-ray-induced DNA damage. Integrated mRNA-seq and small RNA-seq across DJ-1 states in HEK293 cells revealed a striking divergence in miRNA plasticity. DJ-1 depletion by siRNA produced minimal miRNA remodeling, with only 34 (6.3%) miRNAs differentially expressed after irradiation. In contrast, elevated DJ-1 markedly increased miRNA plasticity: irradiation altered [~]37% of detectable miRNAs, accounting for [~]90% of miRNA reads, and extensively redistributed the miRNA pool. DJ-1 overexpression was also associated with remodeling of the miRNA regulatory machinery, particularly components involved in miRNA sorting and stability, suggesting feedback regulation of the miRNA pool. Comparison of precursor and mature species revealed substantial uncoupling between transcription and mature miRNA abundance, implicating regulation at the levels of processing, maturation, or stability. Radiation-responsive coding genes in DJ-1-overexpressing cells were relatively depleted of miRNA binding sites, supporting preferential regulation of upstream regulatory nodes rather than the bulk transcriptome. Together, these findings identify DJ-1 as a determinant of post-transcriptional signaling plasticity, enabling dynamic remodeling of the miRNA regulatory state in response to genotoxic stress. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=133 SRC="FIGDIR/small/744036v1_ufig1.gif" ALT="Figure 1000"> View larger version (38K): org.highwire.dtl.DTLVardef@18824aorg.highwire.dtl.DTLVardef@111d8bcorg.highwire.dtl.DTLVardef@ac33b4org.highwire.dtl.DTLVardef@17698d3_HPS_FORMAT_FIGEXP M_FIG C_FIG