Prime-Editing in Marchantia paleacea: Expanding the Genome-Editing Toolbox in Bryophytes
Danilo, B.; Quillien, A.; Rojas-Latorre, C.; Nibani, Z.; Mestre, C.; Delaux, P.-M.; Lauressergues, D.; Neveu, J.
Show abstract
Since the development of CRISPR-based genome editing tools, a number of novel technologies have emerged. This includes Prime-Editing that acts as a search and replace genome editing tool. Prime-Editing has been deployed across multiple clades, including in a few flowering plants. Here, we report on the development of an efficient Prime Editor (PE) for the model bryophyte Marchantia. Initial tests were conducted on Acetolactate Synthase as a target and revealed an average efficiency above 40%. The system has been developed in the GoldenGate cloning system, facilitating construct design. The development of PE in Marchantia expands the Genome-Editing tools available for this emerging model in plant biology.
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