A Polarized Histamine-GABA Core-Rim Architecture within Synaptic Vesicles
Fujiwara, K.
Show abstract
Neuroscience traditionally assumes that amino acid transmitters occupy clear synaptic vesicles, whereas monoamines reside in dense-core vesicles. Using a glutaraldehyde-NaBH epitope-engineering platform enabling ultrastructural detection of small amines, we identify a polarized histamine-GABA vesicular organization within conventional GABAergic vesicles. Quantitative electron microscopy demonstrates histamine condensed into a dense intraluminal core, while complementary GABA immunolabeling supports the localization of GABA toward the vesicle periphery, consistent with a membrane-proximal rim. This conserved architecture across central, autonomic, and endocrine GABAergic systems provides a structural framework for temporally differentiated inhibitory signaling, challenges the clear-versus-dense-core vesicle paradigm, and establishes a unified principle for dual-transmitter architecture. One-sentence summaryUsing glutaraldehyde-NaBH4-based ultrastructural analysis, we identified a novel "core histamine-rim GABA" vesicular architecture within GABAergic neurons, fundamentally redefining traditional models of dual-transmitter co-packaging and release dynamics.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Cryo-correlative light and electron tomography of dopaminergic axonal varicosities reveals non-synaptic modulation of cortico-striatal synapses 94%
- Structural Basis for the Clamping and Ca2+ Activation of SNARE-mediated Fusion by Synaptotagmin 94%
- Two successive oligomeric Munc13 assemblies scaffold vesicle docking and SNARE assembly to support neurotransmitter release 94%
Similar papers in this journal
- Distinct nanoscale calcium channel and synaptic vesicle topographies contribute to the diversity of synaptic function 96%
- Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity 94%
- Astrocyte-Secreted Neurocan Controls Inhibitory Synapse Formation and Function 93%
Similar papers in this journal
- Metabolic Regulation of Single Synaptic Vesicle Exo- and Endocytosis in Hippocampal Synapses 93%
- Ca2+-dependent phosphodiesterase 1 regulates the plasticity of striatal spiny projection neuron glutamatergic synapses 92%
- Neurons and astrocytes have distinct organelle signatures and responses to stress 92%
Similar papers in this journal
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.