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Dopamine dips during unrewarded actions promote punishment-resistant reward seeking

Mwenda, N.; Nadel, J. A.; Shen, T.; Seiler, J. L.; Lerner, T. N.

2026-08-03 neuroscience
10.64898/2026.08.01.742224 bioRxiv
Show abstract

Punishment-resistant reward-seeking, a hallmark of addiction, is less prominent in females than males. We found that chronic estradiol manipulations increased punishment resistance in female mice without increasing dopamine peaks on rewarded actions as expected, instead exaggerating dopamine dips on unrewarded actions; optogenetically mimicking these dips accelerated punishment resistance in females and males. These results suggest dopamine dips suppress learning from unrewarded actions, consistent with policy-based accounts of dopamine function.

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